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Abstract:
An iridovirus, Apis iridescent virus (AIV), isolated from sick adult specimens of Apis cerana (Hymenoptera) from Kashmir, closely resembles iridescent viruses from Tipula and Sericesthis spp. (TIV and SIV). However, AIV is only distantly related serologically to TIV and SIV and is even more remotely related to several other similar viruses that were tested in tube precipitation tests with intact particles. AIV multiplies in Apis mellifera, forming cytoplasmic iridescent crystalline aggregates in several tissues, but unlike all the other iridoviruses tested, it failed to multiply in Galleria mellonella.
Insights
A novel Apis iridescent virus (AIV) was discovered in honeybees. This virus replicates in European honeybees but not in wax moths, distinguishing it from related iridoviruses.
Area of Science:
- * Virology
- * Entomology
- * Insect Pathology
Background:
- * Apis iridescent virus (AIV) is an iridovirus identified in adult Apis cerana bees from Kashmir.
- * AIV shares morphological similarities with Tipula iridescent virus (TIV) and Sericesthis iridescent virus (SIV).
Purpose of the Study:
- * To characterize the Apis iridescent virus (AIV) isolated from Apis cerana.
- * To compare AIV serologically and biologically with other known iridoviruses.
- * To determine the host range and replication capabilities of AIV.
Main Methods:
- * Isolation and identification of the iridovirus from diseased Apis cerana.
- * Serological testing using tube precipitation tests with intact viral particles.
- * Inoculation experiments in Apis mellifera (European honeybees) and Galleria mellonella (wax moths) to assess replication.
Main Results:
- * AIV, isolated from Apis cerana, shows close resemblance to TIV and SIV.
- * Serological tests indicate AIV is distantly related to TIV and SIV, and even more remotely to other tested iridoviruses.
- * AIV successfully replicates in Apis mellifera, forming cytoplasmic crystalline aggregates.
- * Unlike other tested iridoviruses, AIV failed to replicate in Galleria mellonella.
Conclusions:
- * AIV represents a distinct iridovirus species with a specific host range.
- * The inability of AIV to replicate in Galleria mellonella differentiates it from other known iridoviruses.
- * Further research is needed to understand the pathogenicity and ecological impact of AIV in bee populations.