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Related Experiment Videos

The effect of PHA-activated MN-cell supernatants on polymorphonuclear leucocyte function.

R Lomnitzer, A Glover, A R Rabson

    Clinical and Experimental Immunology
    |September 1, 1977
    PubMed
    Summary

    PHA-activated mononuclear-cell supernatants enhance polymorphonuclear-leucocyte phagocytosis and surface charge but inhibit motility. Leucocyte inhibitory factor (LIF) activity loss suggests PMN interaction is key.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Mononuclear-cell (MN) supernatants can modulate polymorphonuclear-leucocyte (PMN) functions.
    • Phytohemagglutinin (PHA) activation is a common method to stimulate MN cells.

    Purpose of the Study:

    • To investigate the effects of PHA-activated MN-cell supernatants on various PMN functions.
    • To assess the role of leucocyte inhibitory factor (LIF) in these interactions.

    Main Methods:

    • Treatment of PMN with PHA-activated MN-cell supernatants.
    • Assessment of PMN electrophoretic mobility, directional motility, chemotaxis, phagocytic activity, nitroblue tetrazolium (NBT) reduction, glucose oxidation, and bactericidal capacity.
    • Absorption experiments using PMN, human MN cells, and guinea-pig peritoneal exudate cells to determine LIF activity.

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    Main Results:

    • Supernatants increased PMN negative surface charge and phagocytic activity, NBT reduction, and hexosemonophosphate shunt activity.
    • PMN directional motility was inhibited by sustained supernatant exposure.
    • Leucocyte inhibitory factor (LIF) activity was lost from supernatants after absorption with PMN, indicating specific interaction and acquired inhibition of PMN migration.

    Conclusions:

    • PHA-activated MN-cell supernatants significantly alter PMN functions, enhancing some and inhibiting others.
    • Leucocyte inhibitory factor (LIF) in these supernatants plays a role in modulating PMN migration, with specific interaction involving PMN cells.