Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

On newborn hypothalamic phenylethanolamine-N-methyltransferase.

C Torda

    Enzyme
    |January 1, 1977
    PubMed
    Summary

    Stress significantly increases Phenylethanolamine-N-methyltransferase (PNMT) activity in rat hypothalami. This increase, driven by protein synthesis and signaling pathways, is potentiated by prolonged stress exposure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Studies on myasthenia gravis; apparent curare-like effect of compounds that decrease acetylcholine synthesis.

    Federation proceedings·2010
    Same author

    Effect of penicillin and sulfonamides on acetylcholine synthesis and on the response of muscle to acetylcholine and potassium.

    The Journal of laboratory and clinical medicine·2010
    Same author

    Effect of alkaloids on acetylcholine synthesis.

    Archives of biochemistry·2010
    Same author

    Effect of epinephrine and physostigmine on the response of striated muscle to acetylcholine and potassium.

    The American journal of physiology·2010
    Same author

    Apparent curare effect of substances that decrease acetylcholine synthesis.

    The American journal of physiology·2010
    Same author

    Effect of inhibition of glycolysis and compounds related to glycolysis on acetylcholine synthesis.

    The Journal of biological chemistry·2010

    Area of Science:

    • Neuroendocrinology
    • Molecular Biology
    • Stress Physiology

    Background:

    • Phenylethanolamine-N-methyltransferase (PNMT) is a key enzyme in catecholamine synthesis.
    • Understanding PNMT regulation is crucial for comprehending stress responses.

    Purpose of the Study:

    • To investigate the developmental changes in rat hypothalamic PNMT activity.
    • To determine the impact of recurrent stress on PNMT activity.
    • To elucidate the molecular mechanisms underlying stress-induced PNMT potentiation.

    Main Methods:

    • Assay of PNMT activity in rat hypothalami at different developmental stages.
    • Exposure of rats to recurrent stressful situations.
    • Analysis of hypothalamic tissue cultures with and without the PNMT inhibitor SK&F 64139.
    • Measurement of cyclic AMP and cyclic GMP levels.

    Main Results:

    • PNMT activity is present at birth and increases postnatally.
    • Recurrent stress significantly elevates hypothalamic PNMT activity.
    • Stress-induced PNMT increase involves enhanced protein synthesis and potentiation by local factors, including ACTH, cyclic AMP, and cyclic GMP.
    • SK&F 64139 inhibited stress-induced PNMT activity in tissue cultures.

    Conclusions:

    • Hypothalamic PNMT activity exhibits developmental regulation and is sensitive to stress.
    • Stress potentiates PNMT activity through a signaling cascade involving ACTH and cyclic nucleotides, leading to increased protein synthesis.
    • This pathway highlights a molecular mechanism linking stress to altered neurochemical function.

    Related Experiment Videos