Related Experiment Videos
Streptococcus milleri in the appendix
Abstract:
The appendix was investigated as a possible habitat of Streptococcus milleri. Both normal and inflamed appendices were examined and the isolation rates compared. S. milleri was present in a quarter of the normal appendices and more than half of those associated with apendicitis--a difference that was statistically highly significant. The isolation rates throughout were indepencent of age. There was a pronounced connection between the presence of S. milleri in the appendix and the purulent manifestations of appendicitis. S. milleri was isolated from other abdominal sites associated with appendicitis. The frequency of isolation was increased by culture in an enrichment broth containing nalidixic acid and sulphadimidine.
Insights
Streptococcus milleri is frequently found in inflamed appendices, suggesting it plays a role in appendicitis. This bacterium was also detected in other abdominal sites during appendicitis cases.
Area of Science:
- Microbiology
- Gastroenterology
- Surgical Pathology
Background:
- The appendix's role in harboring bacteria is not fully understood.
- Streptococcus milleri is a known opportunistic pathogen.
Purpose of the Study:
- To investigate the appendix as a potential habitat for Streptococcus milleri.
- To compare the isolation rates of S. milleri in normal versus inflamed appendices.
Main Methods:
- Examination of both normal and inflamed appendix tissues.
- Bacterial culture using enrichment broth with nalidixic acid and sulphadimidine.
Main Results:
- S. milleri was isolated from 25% of normal appendices and over 50% of inflamed appendices.
- A significant correlation was observed between S. milleri presence and purulent appendicitis.
- S. milleri was also found in other abdominal sites associated with appendicitis.
Conclusions:
- The appendix serves as a significant reservoir for Streptococcus milleri.
- S. milleri is strongly associated with the development and purulent manifestations of appendicitis.