Related Experiment Video

Updated: Aug 5, 2026

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
13:12

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

Published on: April 4, 2011

Characterization of a low molecular weight antigenic protein from the envelope of influenza virus

E A Eckert

    Journal of Bacteriology
    |November 1, 1966
    PubMed

    Insights

    Researchers characterized a low molecular weight antigenic protein from the influenza virus envelope. This protein reacted with specific antisera but did not agglutinate erythrocytes.

    Area of Science:

    • Virology
    • Immunochemistry
    • Protein Chemistry

    Background:

    • Influenza virus envelope proteins are crucial for viral structure and antigenicity.
    • Understanding the properties of individual viral proteins aids in vaccine development and antiviral strategies.

    Purpose of the Study:

    • To characterize a low molecular weight antigenic protein isolated from the influenza virus envelope.
    • To investigate the subunit structure and antigen-binding capabilities of this protein.

    Main Methods:

    • Solubilization of viral protein using urea and dithiothreitol (DTT).
    • Sedimentation analysis in the presence of urea-DTT and after dialysis.
    • Serological assays including complement fixation and blocking-antigen tests.

    Main Results:

    • A low molecular weight antigenic protein was successfully solubilized.
    • The protein existed as 2S subunits in urea-DTT, reassociating to a 4S state upon dialysis.
    • The protein reacted with strain-specific antisera but did not exhibit hemagglutination activity.

    Conclusions:

    • The characterized protein is a distinct antigenic component of the influenza virus envelope.
    • Its subunit behavior suggests specific structural properties influencing its antigenicity.
    • The findings contribute to the understanding of influenza virus antigen structure and immune response.

    More Related Videos

    Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses08:10

    Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

    Published on: January 15, 2020

    Subnanometer-Resolution Structural Determination of Hemagglutinin from Cryo-Electron Tomography of Influenza Viruses08:19

    Subnanometer-Resolution Structural Determination of Hemagglutinin from Cryo-Electron Tomography of Influenza Viruses

    Published on: November 7, 2025

    Related Concept Videos

    Leaky Scanning02:28

    Leaky Scanning

    During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
    Influenza01:27

    Influenza

    Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
    Inhibitors Of Virion Release01:25

    Inhibitors Of Virion Release

    Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...