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Partial purification and electron microscopy of virus in the EB-3 cell line derived from a Burkitt lymphoma
Science (New York, N.Y.)
|May 20, 1966
Abstract:
The virus in the EB-3 cell line derived from a Burkitt lymphoma was partially purified by digestion with proteolytic enzyme and by density-gradient centrifugation on potassium tartrate. Electron microscope studies of both sectioned and negatively stained samples suggest that the virus belongs to the herpes-virus group, although the exact number of capsomeres was not unequivocally established.
Insights
Researchers partially purified a virus from EB-3 Burkitt lymphoma cells. Electron microscopy suggests it belongs to the herpes-virus group, though capsomere count remains uncertain.
Area of Science:
- Virology
- Cell Biology
- Oncology
Background:
- Burkitt lymphoma is a cancer of B-cells.
- EB-3 is a cell line derived from Burkitt lymphoma.
- Viruses are implicated in some cancers.
Purpose of the Study:
- To characterize a virus present in the EB-3 cell line.
- To determine the viral group classification.
Main Methods:
- Partial purification using proteolytic enzyme digestion.
- Density-gradient centrifugation using potassium tartrate.
- Electron microscopy (sectioned and negatively stained samples).
Main Results:
- The virus was successfully partially purified.
- Electron microscopy indicated the virus is likely a herpes-virus.
- The precise number of capsomeres could not be definitively determined.
Conclusions:
- The virus in the EB-3 cell line shares characteristics with herpes-viruses.
- Further studies are needed to confirm the exact viral classification and structure.