Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial configuration of mRNA 5'-terminus.

C H Kim, R H Sarma

    Nature
    |November 17, 1977
    PubMed
    Summary

    Nuclear magnetic resonance reveals the 5-prime cap structure of messenger RNA (mRNA) adopts a stacked conformation. This spatial arrangement of bases is crucial for mRNA function and regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Expression of secreted and membrane-bound mucins in the airways of piglets experimentally infected with Mycoplasma hyopneumoniae.

    Veterinary journal (London, England : 1997)·2011
    Same author

    Expression of RUNX3 in skin cancers.

    Clinical and experimental dermatology·2011
    Same author

    Malignant melanoma of the vulva: an extension of cutaneous melanoma?

    Gynecologic oncology·2011
    Same author

    Molecular inflammation as an underlying mechanism of the aging process and age-related diseases.

    Journal of dental research·2011
    Same author

    Comparative efficacy of commercial Mycoplasma hyopneumoniae and porcine circovirus 2 (PCV2) vaccines in pigs experimentally infected with M. hyopneumoniae and PCV2.

    Vaccine·2011
    Same author

    Target structure induced suppression of the ionization cross section for very low energy antiproton-hydrogen collisions.

    Physical review letters·2011

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Background:

    • The 5'-terminus of messenger RNA (mRNA) features a unique cap structure, methylated at the guanosine (m7G).
    • This cap structure plays critical roles in mRNA stability, export, and translation initiation.
    • Understanding the spatial conformation of the cap is essential for elucidating its functional mechanisms.

    Purpose of the Study:

    • To determine the three-dimensional spatial configuration of the mRNA 5'-cap structure (m7G5'ppp5'Am).
    • To investigate how the bases within the cap structure are arranged in space.
    • To correlate the spatial conformation with potential functional implications.

    Main Methods:

    • Nuclear magnetic resonance (NMR) spectroscopy was employed to analyze the mRNA cap structure.
    • Analysis included detailed examination of coupling constants and chemical shift trends.
    • Ring current effects were considered to interpret the spatial arrangement of the bases.

    Main Results:

    • The 5'-terminus m7G5'ppp5'Am of mRNA exhibits a distinct spatial configuration.
    • The bases within the cap structure form stacked arrays.
    • NMR data provided detailed insights into the specific conformation adopted by the cap.

    Conclusions:

    • The mRNA 5'-cap structure is not linear but adopts a specific, stacked spatial arrangement.
    • This conformation likely influences interactions with proteins and other nucleic acids.
    • Further studies can explore the functional consequences of this determined spatial configuration.

    Related Experiment Videos