Photometric extraction method for determination of cefalotin

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis
• Spectrophotometry

Background:

• Accurate quantification of antibiotics like cefalotin is crucial for quality control and therapeutic monitoring.
• Existing methods for cefalotin determination may lack selectivity or require complex procedures.
• Development of a simple, rapid, and precise assay is needed for routine analysis.

Purpose of the Study:

• To develop and validate a selective, accurate, and precise photometric method for cefalotin determination.
• To utilize the degradation product of cefalotin for quantitative analysis.
• To establish a cost-effective and efficient analytical technique.

Main Methods:

• Hydrolysis of cefalotin in concentrated sulfuric acid at 100°C for 15 minutes.
• Photometric determination of the resulting 2-thienylacetic acid using ninhydrin in acidic solution.
• Extraction of the colored product with chloroform and measurement of absorbance at 423 nm.

Main Results:

• The method demonstrated high selectivity, with minimal interference from related substances and cefalexin.
• Accurate and precise quantification was achieved, with a coefficient of variation ranging from 0.57% to 0.88% and a relative error from 0.41% to 0.64%.
• The assay is simple and fast, suitable for routine laboratory use.

Conclusions:

• A novel and robust photometric method for cefalotin quantification has been successfully developed.
• The method's selectivity, accuracy, and precision make it a valuable tool for pharmaceutical analysis.
• This assay provides an efficient alternative for determining cefalotin in various sample matrices.