A hybrid technique for staining meiotic nuclei of Basidiomycetes

A hybrid nuclear staining procedure used on the basidiomycete Schizophyllum commune produced squash preparations of sufficient density for effective brightfield photomicrography.

Tissure is fixed in Newcomer's solution, rehydrated in a graded ethanol series, rinsed, hydrolyzed in 1 N HC1 at 60 C, washed, mordanted in 4% iron alum, rinsed again, and stained with aceto-iron-hematoxylin prior to mounting and squashing.