Diffusivity measurements of human methemoglobin

Experimental measurements of the diffusion coefficient of human methemoglobin were made at 25 degrees C with a modified Stokes diaphragm diffusion cell. A Millipore filter was used in place of the ordinary fritted disc to facilitate rapid achievement of steady state in the diaphragm. Methemoglobin concentrations varied from approximately 5 g/100 ml to 30 g/100 ml. The diffusion coefficient in this range decreased from 7.5 x 10(-7) cm(2)/sec to 1.6 x 10(-7) cm(2)/sec.