Comparative studies of the protein binding of digoxin and its metabolites

The protein binding of Digoxin (D), Dihydrodigoxin (DH), Digoxigenin Bisdigitoxoside (DB) and Digoxigenin Monodigitoxoside (DM) was concentration independent and unaltered by the presence of high concentrations of the analogous compounds. The respective percentage of protein binding for D, DH, DB and DM were 22%, 22%, 23% and 27%.