Energy-dependent uptake of arsenite by rat liver mitochondria

Uptake of arsenite by rat liver mitochondria is energy-dependent, as shown by comparing values without and with either uncoupling agent or respiratory inhibitor present. The uptake is inhibited by mersalyl and N-ethylmaleimide, which can be used as 'stopping' agents to obtain uptake kinetics. At 20 degrees C the process is nearly complete in 1 min. The relation between the quantity in the energized mitochondria and the applied concentration corresponds to at least two different modes of binding of the arsenite. Competition occurs between arsenite and other anions (for example, phosphate) for intramitochondrial accumulation.