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alpha-Pinene metabolism by Pseudomonas putida.

N J Tudroszen, D P Kelly, N F Millis

    The Biochemical Journal
    |November 15, 1977
    PubMed
    Summary
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    Researchers identified new intermediate compounds in alpha-pinene metabolism using Pseudomonas putida PIN11. These novel compounds, 3-isopropylbut-3-enoic acid and (zeta)-2-methyl-5-isopropylhexa-2,5-dienoic acid, advance our understanding of microbial degradation pathways.

    Area of Science:

    • Microbial metabolism
    • Biochemistry
    • Organic chemistry

    Background:

    • Alpha-pinene is a prevalent cyclic monoterpene found in essential oils.
    • Understanding its microbial degradation is crucial for bioremediation and biotechnology.
    • Pseudomonas putida PIN11 is known for its ability to metabolize various aromatic compounds.

    Purpose of the Study:

    • To identify novel intermediates in the metabolic pathway of alpha-pinene by Pseudomonas putida PIN11.
    • To characterize the chemical structures of these newly detected compounds.
    • To elucidate the initial steps of alpha-pinene biodegradation.

    Main Methods:

    • Utilized metabolically altered mutants of Pseudomonas putida PIN11.
    • Introduced acrylate to the culture medium to probe metabolic pathways.

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  • Employed analytical techniques for the detection and characterization of intermediates.

    • Main Results:

    • Successfully detected and characterized two novel putative intermediates.
    • Identified 3-isopropylbut-3-enoic acid as a metabolic product.
    • Identified (zeta)-2-methyl-5-isopropylhexa-2,5-dienoic acid as another metabolic product.

    Conclusions:

    • The study reveals previously unknown steps in alpha-pinene metabolism.
    • The identified intermediates provide insights into the enzymatic transformations involved.
    • These findings contribute to the understanding of microbial degradation of terpenes.