A method for the analysis of chlorhexidine in serum

Area of Science:

• Analytical Chemistry
• Clinical Chemistry
• Pharmacology

Background:

• Chlorhexidine is a widely used antiseptic with potential for systemic absorption.
• Accurate quantification of chlorhexidine in biological matrices is essential for safety and efficacy.
• Existing methods may lack the sensitivity or practicality for routine clinical use.

Purpose of the Study:

• To develop and validate a sensitive colorimetric assay for chlorhexidine determination.
• To establish the limit of detection and assess the method's suitability for clinical samples.

Main Methods:

• Elaboration of a colorimetric method for chlorhexidine quantification.
• Analysis of serum or plasma samples using the developed method.
• Determination of the limit of detection (LOD) using standard laboratory procedures.

Main Results:

• A robust colorimetric method for chlorhexidine determination in serum/plasma was successfully developed.
• The method achieved a limit of detection of 25 ng/ml.
• The assay requires a sample volume of 10 ml of serum or plasma.

Conclusions:

• The developed colorimetric method provides a sensitive and reliable means for quantifying chlorhexidine in biological fluids.
• This assay is suitable for monitoring chlorhexidine levels in clinical settings.
• Further validation may be warranted for specific applications.