Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphocyte stimulation by soluble subcellular fractions.

G D Pegrum, E A Thompson, C M Lewis

    Tissue Antigens
    |April 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

    Physical review letters·2026
    Same author

    Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Observation of tt[over ¯] Production in Pb+Pb Collisions at sqrt[s_{NN}]=5.02  TeV with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Search for Dark Matter Produced in Association with a Dark Higgs Boson in the bb[over ¯] Final State Using pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Spatially defined intratumoral immune biomarkers predict recurrent versus second primary tumors in non-small cell lung cancer.

    Precision clinical medicine·2025

    Nuclear and cytoplasmic fractions from cells can stimulate healthy leucocytes, influencing immune responses. This reactivity is linked to Human Leukocyte Antigen (HLA) activity, though less potent than standard tests.

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Nuclear material's role in immune cell modulation is complex.
    • Previous studies have explored cellular interactions, but fraction-specific effects remain less understood.

    Purpose of the Study:

    • To investigate the immunomodulatory effects of nuclear and cytoplasmic fractions on healthy lymphocytes.
    • To correlate cellular fraction activity with Human Leukocyte Antigen (HLA) expression.

    Main Methods:

    • Comparison of whole cells, nuclear fractions, and cytoplasmic fractions on lymphocyte cultures.
    • Assessment of lymphocyte reactivity and HLA activity in response to different cellular components.
    • Utilized mixed lymphocyte culture (MLC) techniques for comparison.

    Related Experiment Videos

    Main Results:

    • Intact nuclei did not induce reactivity with allogeneic lymphocytes.
    • Specific nuclear and cytoplasmic fractions demonstrated stimulatory effects on lymphocytes.
    • Fraction-induced stimulation correlated with peaks in HLA activity.
    • The observed stimulation was less pronounced than in conventional one-way MLC tests.

    Conclusions:

    • Certain nuclear and cytoplasmic fractions possess immunomodulatory properties.
    • HLA activity is a key factor in the stimulatory capacity of these fractions.
    • Fraction-based immune stimulation offers a potential area for further research, distinct from whole-cell interactions.