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Updated: Aug 14, 2026

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 2, 2011
Specificity of Hpa II and Hae III DNA methylases
Abstract:
The methylases M.HaeIII and M.HpaII recognize the tetranucleotide sequences [Formula: see text] and [Formula: see text] respectively, in DNA, and transfer a methyl group from S-adenosylmethionine to the 5-position of cytosine on each strand as indicated by the asterisks. Restriction endonuclease R.HaeIII does not cleave the methylated sequence [Formula: see text] but can cleave [Formula: see text] in which methylation is introduced on the unnatural external cytosine positions. Similarly, R.HpaII does not cleave [Formula: see text] but can cleave [Formula: see text].Images
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