[Gel filtration chromatography of tick-borne encephalitis virus on macropore glass]

The capacity of some chemically treated porous glass to adsorb tissue culture virus of tick-borne encephalitis was studied. Chemical binding of albumin and succinanhydride with the porous glass matrix was found to eliminate virion adsorption on the carrier surface. This permitted to carry out gel filtration chromatography of several virus strains and to obtain highly purified preparations of tick-borne encephalitis virus. Gel filtration on modified porous glass with different pore sizes was used for the determinations of cromatographic radius of virus particle.