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Leukemogenic activity of ether-extracted Rauscher leukemia virus
Abstract:
After rapid multiple extractions of mouse plasma virus with ether, the aqueous solution contained viral nucleoids that were infectious when inoculated intracranially into newborn BALB/c mice. The infectivity associated with the ether extract was not neutralized by the specific antibody prepared against the whole virus. No intact virus has been seen in these preparations. Treatment with ether completely removed the virus envelope from the particle and produced an apparently homogeneous preparation of viral nucleoids. After the extractions with ether, leukemogenic activity was inactivated by exposure to ribonuclease. The leukemogenic activity of the many-passaged Rauscher virus that has been propagated in tissue culture and that has low infectivity was also retained, and, in two experiments in which material was inoculated intracranially into mice, this activity appeared to have been enhanced by multiple extractions with ether.
Insights
Ether extraction of mouse plasma virus yielded infectious viral nucleoids, enhancing leukemogenic activity. This process removed the virus envelope, retaining infectivity and potentially increasing Rauscher virus leukemogenic potential.
Area of Science:
- Virology
- Oncology
Background:
- Rauscher virus is a known leukemogenic agent in mice.
- Understanding virus structure and infectivity is crucial for disease research.
Purpose of the Study:
- To investigate the effects of ether extraction on mouse plasma virus.
- To characterize the infectivity and leukemogenic potential of viral nucleoids.
Main Methods:
- Multiple ether extractions of mouse plasma virus.
- Intracranial inoculation of extracted material into newborn BALB/c mice.
- Treatment with ribonuclease to assess leukemogenic activity.
Main Results:
- Ether extraction yielded infectious viral nucleoids, free of intact virus and envelope.
- Infectivity of ether extracts was not neutralized by specific antibodies.
- Leukemogenic activity was inactivated by ribonuclease but retained/enhanced in Rauscher virus.
Conclusions:
- Ether extraction effectively isolates infectious viral nucleoids.
- Viral nucleoids possess leukemogenic potential.
- Ether extraction may enhance the leukemogenic activity of certain viruses like Rauscher virus.