Simple medium for the selective isolation of Bacteroides and related organisms, and their occurrence in sewage

A medium composed of 0.009% sodium azide, 0.07% sodium deoxycholate, and 0.0007% ethyl violet in Brain Heart Infusion Agar (Difco) and a process of incubation in an atmosphere of 90% N(2) and 10% CO(2) for the selective isolation of certain members of the intestinal bacteroides are described. The medium appears to select predominantly members of the genus Bacteroides and a few of the genus Sphaerophorus. A survey of the occurrence of these organisms in sewage and various stages of sewage treatment indicates that they survive complete sewage treatment in low numbers and that their rate of decline parallels that of the coliforms. Large numbers were recovered from sludge digestion tanks, suggesting a possible role in the anaerobic breakdown of organic matter.