Multiple cholinesterases in rat brain homogenate

Two kinetic evidences revealed that two cholinesterase forms are present in rat brain homogenate and involved in the hydrolysis of acetylthiocholine. These two kinetic forms have different affinity to acetylthiocholine (34 fold), and different sensitivity to carbofuran, 2,2-dimethyl 2,3-dihadro-7-benzofuranyl methylcarbamate (33 fold). The proportion of the enzyme in each kinetic form is 0.52 form I and 0.48 form II.