Duration of the antifertility effect on glycerol in the chicken vagina

To determine the duration of the antifertility effect of glycerol in the chicken's vagina, groups of 15 Leghorn-type hens were each given 0.1 ml. of a 2.076 M glycerol solution at time intervals from 0 to 10 minutes before and after insemination with 0.1 ml. of pooled semen. Eggs were set weekly and candled at 7 days of incubation to determine fertility. Fertility of eggs collected during days 5--7 was significantly (P less than .01) reduced by the glycerol solution when it was administered between 5 minutes before and 10 minutes after insemination. The antifertility effect disappeared within 10 minutes after glycerol deposition.