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Erythropoietic activity and interferon production in LCM virus-infected nude mice
Abstract:
The distinct haemopoietic lesions induced in ordinary mice during acut LCM virus infection are not mediated either by immunopathological mechanisms or by a direct cytopathogenetic effect of the virus. Medication has recently been artibuted to the high interferon activity found in mice with acute LCM virus infection. In this work, erythropoietic activity and interferon production have been studied in LCM virus-infected nude mice. Compared with ordinary mice, nude mice displayed a more moderate suppression of erythropoiesis and a very poor interferon response. Erythropoietic activity of thymus-transplanted nude mice was not significantly suppressed during the infection, and interferon responsiveness was not restored either by thymus-transplantation or by transfer of large numbers of spleen lymphocytes. The observations support the hypothesis that interferon plays a decisive role in the induction of haemopoietic diorders of mice with acute LCM virus infection. The puzzling question as to why nude mice and reconstituted nude mice are incapable of mounting a normal interferon response is discussed briefly.
Insights
High interferon activity during LCM virus infection causes hematopoietic lesions in mice. Nude mice show reduced lesions and poor interferon response, suggesting interferon
Area of Science:
- Immunology
- Virology
- Hematology
Background:
- Acute LCM virus infection in mice causes hematopoietic lesions.
- These lesions are not caused by immunopathology or direct viral effects.
- High interferon activity is suspected to mediate these lesions.
Purpose of the Study:
- To investigate the role of interferon in LCM virus-induced hematopoietic lesions.
- To compare erythropoietic activity and interferon production in LCM virus-infected nude mice versus ordinary mice.
Main Methods:
- Studied erythropoiesis and interferon production in LCM virus-infected nude mice.
- Compared results with ordinary mice.
- Investigated effects of thymus transplantation and spleen lymphocyte transfer in nude mice.
Main Results:
- Nude mice exhibited moderate erythropoiesis suppression and a poor interferon response compared to ordinary mice.
- Thymus-transplanted nude mice showed no significant suppression of erythropoiesis.
- Neither thymus transplantation nor spleen lymphocyte transfer restored interferon responsiveness in nude mice.
Conclusions:
- Interferon plays a decisive role in inducing hematopoietic disorders during acute LCM virus infection.
- Nude and reconstituted nude mice are incapable of mounting a normal interferon response, warranting further investigation.