[In vitro behavior of 14C-lindane in blood]

Almost 55% of lindane (gamma-HCH) which had been applied in vitro are removed by simple hexane extraction of the blood; if blood fractions are extracted, a total rate of 95% results. The partition of gamma-HCH between the plasma and the erythrocytes is approximately I:I; when HCH erythrocytes are incubated in fresh plasma, almost 60% of erythrocyte HCH pass into the plasma. The erythrocytes absorb nearly 90% of the HCH contained in an aqueous medium. Haemolysis showed that HCH is most probably transferred through the membrane. The partition of HCH between the cytoplasm and the membrane fraction ranges from 3:7 to 7:3. TCA precipitates almost 90% of erythrocyte HCH together with the protein. The absorbing capacity of the erythrocytes could be followed up to nearly 0.4-10(-6)M/ml of blood. Cadmium increases, lead decreases the binding of HCH to the erythrocytes; mercury and ethanol exert but small effects.