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Related Experiment Videos

Cellular calcium mobilization.

E E Daniel

    Journal of Cardiovascular Pharmacology
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Smooth muscles utilize intracellular calcium (Ca) stores for contraction. Different Ca stores, released by agonists or caffeine, exhibit distinct refill and release characteristics, suggesting varied cellular locations and functions.

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    Area of Science:

    • Cellular Physiology
    • Muscle Contraction
    • Calcium Signaling

    Background:

    • Intracellular calcium (Ca) stores are crucial for smooth muscle contraction.
    • Distinct Ca stores exist, differing in release mechanisms and cellular localization.
    • Agonist- and caffeine-induced Ca release pathways present unique characteristics.

    Purpose of the Study:

    • To characterize different intracellular calcium stores in smooth muscles.
    • To investigate the properties of agonist- versus caffeine-releasable Ca stores.
    • To explore the existence and function of a recycling Ca store.

    Main Methods:

    • Mobilization of intracellular Ca stores using agonists and caffeine.
    • Experiments in Ca2+-free solutions to assess store refilling and recycling.

    Related Experiment Videos

  • Saponin treatment to probe plasma membrane integrity and store localization.

    • Main Results:

    • Agonist-released Ca stores require high agonist concentrations, do not recycle Ca, refill rapidly, and disappear with saponin treatment.
    • Caffeine-released Ca stores refill slowly, are not always depleted with agonists, and persist after saponin treatment.
    • A recycling Ca store, releasable by agonists, can be unmasked in Ca2+-free solutions, exhibiting slower Ca release and reuptake.

    Conclusions:

    • Smooth muscles possess multiple intracellular Ca stores with distinct properties.
    • Agonist-sensitive stores may be located near the plasma membrane, while caffeine-sensitive stores are likely in the endoplasmic reticulum.
    • A recycling Ca store mechanism exists, requiring specific cellular processes to maintain Ca homeostasis during prolonged stimulation.