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Alkaline phosphatase activity of the mouse
Summary
Plasma alkaline phosphatase (PAP) activity in mice is low and varies by sex and husbandry. Two PAP isoenzymes likely originate from bone and liver, identified through electrophoresis and inhibitor studies.
Area of Science:
- Biochemistry
- Animal Physiology
- Enzymology
Background:
- Plasma alkaline phosphatase (PAP) activity in young adult CD mice is notably lower than in rats.
- Significant sex-based differences exist, with lower PAP activity observed in male mice compared to females.
- Variability in PAP was investigated across diverse husbandry conditions, including caging, diet, and specific pathogen-free (SPF) environments.
Purpose of the Study:
- To characterize plasma alkaline phosphatase (PAP) activity in CD mice.
- To investigate factors influencing PAP variability, such as sex and husbandry.
- To identify the origins of PAP isoenzymes in mice.
Main Methods:
- Assessment of PAP activity in mice under various husbandry conditions.
- Comparison of PAP activity between fed and fasted mice.
- Acrylamide electrophoresis and inhibitor studies to differentiate PAP isoenzymes and compare them with organ extracts.
Main Results:
- PAP activity was found to be similar in fed and fasted mice.
- Electrophoresis and inhibitor profiles suggested the presence of two PAP isoenzymes.
- Heat inhibition further supported the distinction between these isoenzymes.
Conclusions:
- The two identified PAP isoenzymes likely originate from bone and liver sources.
- PAP activity in mice is influenced by sex and husbandry conditions.
- Further research can utilize these findings for comparative biochemical studies.