Related Experiment Video

Updated: Oct 24, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Synthesis of the Mg antigenic determinant and peptide analogs related to human glycophorin A

B Ferrari, A A Pavia

    International Journal of Peptide and Protein Research
    |November 1, 1984
    PubMed

    Insights

    Researchers synthesized specific peptides related to the rare Mg antigen mutation in the MN blood group system. These synthetic peptides act as artificial antigens and glycosyl transferase acceptors for further study.

    Area of Science:

    • Biochemistry
    • Immunology
    • Hematology

    Background:

    • The Mg antigen is a rare mutation within the MN blood group system.
    • Understanding the molecular basis of blood group antigens is crucial for transfusion medicine and immunology.

    Purpose of the Study:

    • To synthesize specific peptide sequences related to the Mg antigen and other glycophorin variants.
    • To create artificial antigens and glycosyl transferase acceptors for immunological and biochemical studies.

    Main Methods:

    • A stepwise peptide coupling strategy was employed in solution.
    • Synthesis of the amino-terminal pentapeptide of human glycophorin AMg (Leu-Ser-Thr-Asn-Glu).
    • Synthesis of other analogs and pentapeptides representing glycophorin AM, AN, and AMc backbones.

    Main Results:

    • Successfully synthesized the target pentapeptides and analogs.
    • The synthesized compounds are suitable for use as glycosyl transferase acceptors.
    • The compounds can function as artificial antigens in experimental settings.

    Conclusions:

    • The study provides synthetic peptide tools for investigating the Mg antigen and related blood group system components.
    • These synthetic peptides facilitate further research into glycosylation and antigenicity within the MN blood group system.

    More Related Videos

    Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling11:08

    Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

    Published on: February 5, 2018

    Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes13:53

    Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

    Published on: December 19, 2020

    Related Concept Videos

    Proteoglycans01:05

    Proteoglycans

    Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...
    Antigens Involved in Adaptive Immunity01:26

    Antigens Involved in Adaptive Immunity

    An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
    Complete Antigens
    Complete antigens possess both immunogenicity and reactivity.
    Peptidoglycan Synthesis01:28

    Peptidoglycan Synthesis

    Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan biosynthesis begins in...