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Effect of chronic reserpine treatment on the pancreases of neonatal rats
Insights
Chronic reserpine treatment in rat pups impairs pancreatic enzyme secretion, not developmental enzyme accumulation. This suggests reserpine inhibits exocrine function, offering insights into pancreatic disorders.
Area of Science:
- Pediatric Research
- Gastroenterology
- Pharmacology
Background:
- Chronic reserpine in adult rats mimics cystic fibrosis pancreatic changes.
- Cystic fibrosis pancreatic dysfunction often manifests in childhood.
Purpose of the Study:
- To investigate the effects of chronic reserpine treatment on pancreatic function in developing rat pups.
- To determine if reserpine affects pancreatic enzyme accumulation or secretion during suckling.
Main Methods:
- Rat pups treated daily with reserpine or vehicle from day 4 until sacrifice.
- Body and pancreatic weights measured; pancreatic enzyme concentrations (amylase, lipase, trypsinogen) analyzed.
- Intestinal enzyme content assessed; dispersed acini stimulated for amylase release.
Main Results:
- Reserpine pups showed decreased body weight but normal pancreatic weight relative to body weight.
- Pancreatic enzyme concentrations were unaffected, but intestinal enzyme levels decreased, indicating reduced secretion.
- Pancreatic acini from reserpine pups exhibited impaired amylase release upon stimulation.
Conclusions:
- Chronic reserpine treatment in suckling rats does not disrupt pancreatic enzyme developmental accumulation.
- Reserpine treatment significantly inhibits pancreatic exocrine secretion in developing rats.
- This model may provide insights into childhood pancreatic dysfunction.
Abstract:
Chronic reserpine treatment of adult rats results in the accumulation of pancreatic enzymes and reduction of their discharge. These changes are reminiscent of those in cystic fibrosis. Since the majority of cystic fibrosis patients have their pancreatic dysfunction manifested in childhood, we studied chronic reserpine treatment in rat pups. Four-day-old rat pups were given reserpine (50 micrograms/kg intraperitoneally) or vehicle daily until sacrifice. The reserpine group showed significant decreases in body weights at 14 and 21 days of age. Pancreatic weights were also decreased but were of normal weight or increased when normalized against body weights. At 14 and 21 days of age, pancreatic concentrations of amylase, lipase, and trypsinogen showed no difference between reserpine and control pups. At both ages, pancreatic contents of all three enzymes were generally less in the treated pups, but were found to be similar when corrected for body weights. Hydrocortisone treatment of 14-day-old pups caused precocious accumulation of pancreatic enzymes in both reserpine and control groups. Intestinal contents of lipase, trypsin, and amylase were decreased in the reserpine pups at 14 days of age and reached a more significant level at 21 days of age; these data suggest a decrease in the secretion of pancreatic enzymes. Dispersed acini from 14-day-old pups showed a reduced capacity to release amylase as stimulated by carbachol or the octapeptide of cholecystokinin. The results suggested that chronic reserpine treatment of pups in the suckling period did not cause significant disturbance of the developmental accumulation of pancreatic enzymes. A definite inhibition of exocrine secretion was found with reserpine treatment.(ABSTRACT TRUNCATED AT 250 WORDS)