Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

C-polysaccharide in a pneumococcal vaccine.

U B Sørensen, J Henrichsen

    Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology
    |December 1, 1984
    PubMed
    Summary

    Pneumovax vaccine contains cell-wall antigen C-polysaccharide (C-Ps) with varying antigenic determinants and molecular sizes. Lectin affinity chromatography confirmed the presence of cell-wall residues in the C-Ps, indicating potential impurities in the vaccine.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A multi-laboratory evaluation of an enzyme-linked immunoassay quantitating human antibodies to Streptococcus pneumoniae polysaccharides.

    Immunological investigations·2001
    Same author

    [Surgical treatment of severe snoring. Long-term effects].

    Ugeskrift for laeger·2000
    Same author

    Typing of Streptococcus pneumoniae: past, present, and future.

    The American journal of medicine·1999
    Same author

    Relationship between cell surface carbohydrates and intrastrain variation on opsonophagocytosis of Streptococcus pneumoniae.

    Infection and immunity·1999
    Same author

    Does bismuth subgallate have haemostatic effects in tonsillectomy?

    Clinical otolaryngology and allied sciences·1999
    Same author

    [Epidemiology of pneumococcal infections in Europe].

    Presse medicale (Paris, France : 1983)·1998

    Area of Science:

    • Vaccinology
    • Immunochemistry
    • Analytical Chemistry

    Background:

    • Pneumovax is a 14-valent pneumococcal vaccine.
    • Vaccine purity and composition are critical for efficacy and safety.
    • Characterization of vaccine components ensures quality control.

    Purpose of the Study:

    • To analyze the composition of the 14-valent pneumococcal vaccine, specifically the cell-wall antigen C-polysaccharide (C-Ps).
    • To investigate the antigenic properties and molecular heterogeneity of C-Ps.
    • To confirm the presence of cell-wall residues in the C-Ps preparation.

    Main Methods:

    • Quantification of C-polysaccharide (C-Ps) and capsular polysaccharides in Pneumovax.
    • Antigenic characterization of C-Ps using rabbit anti-C-Ps antiserum.
    • Lectin affinity chromatography to detect cell-wall residues.

    Main Results:

    • Pneumovax contains approximately 250 µg/ml of C-Ps and 100 µg/ml of each capsular polysaccharide.
    • Anti-C-Ps antiserum identified four C-Ps components with distinct antigenic determinants and molecular sizes.
    • Lectin affinity chromatography confirmed the presence of cell-wall residues within the C-Ps in the vaccine.

    Conclusions:

    • The 14-valent pneumococcal vaccine contains C-polysaccharide (C-Ps) with significant antigenic heterogeneity.
    • The presence of cell-wall residues indicates potential impurities in the vaccine preparation.
    • The analytical methods employed are suitable for characterizing other polysaccharide preparations.

    Related Experiment Videos