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Characterization of antigenic variation among mink enteritis virus isolates
American Journal of Veterinary Research
|December 1, 1984
Summary
Three antigenic forms of mink enteritis virus were identified. Vaccines from any form protected against all three, indicating broad cross-protection for mink parvovirus.
Area of Science:
- Virology
- Immunology
- Veterinary Medicine
Background:
- Mink enteritis virus (MEV) is a significant pathogen in mink populations.
- Understanding MEV antigenic variation is crucial for vaccine development.
- Monoclonal antibodies are valuable tools for characterizing viral antigens.
Purpose of the Study:
- To identify and characterize antigenic forms of natural field isolates of MEV.
- To evaluate the cross-protective immunity induced by inactivated MEV vaccines.
Main Methods:
- Utilized a panel of monoclonal antibodies against feline panleukopenia virus and canine parvovirus-2 to analyze MEV isolates.
- Employed agar-gel precipitin tests and restriction enzyme mapping to assess antigenic relationships.
- Conducted cross-protection studies using inactivated viral vaccines.
Main Results:
- Identified three distinct antigenic forms of MEV.
- Determined that MEV types 2 and 3 are closely related, but distinct from type 1.
- Demonstrated that inactivated vaccines from any of the three antigenic types conferred homologous and heterologous protection in mink.
Conclusions:
- MEV exhibits antigenic diversity, with at least three variant types.
- A single inactivated MEV vaccine formulation has the potential to protect against all identified antigenic types, simplifying vaccination strategies.