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A blood substitute from hydroxyethyl starch and hemoglobin.

L C Cerny, E L Cerny, J Robach

    Applied Biochemistry and Biotechnology
    |January 1, 1984
    PubMed
    Summary

    Researchers created polymer-bound hemoglobin (Hgb) using hydroxyethyl starch (HES) to improve Hgb retention. Rats transfused with HES-Hgb survived over 10 hours, demonstrating increased Hgb stability and reduced urinary excretion.

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    Area of Science:

    • Biochemistry
    • Polymer Chemistry
    • Hematology

    Background:

    • Hemoglobin (Hgb) is crucial for oxygen transport but has limited in vivo retention time.
    • Hydroxyethyl starch (HES) is a plasma expander that can be chemically modified.
    • Developing stable Hgb solutions is essential for transfusion medicine.

    Purpose of the Study:

    • To synthesize and characterize polymer-bound hemoglobin (Hgb) using hydroxyethyl starch (HES).
    • To evaluate the in vivo efficacy and retention time of HES-Hgb in a rat model.

    Main Methods:

    • Hemoglobin was conjugated with hydroxyethyl starch via the dialdehyde route.
    • Electrophoretic mobility patterns were used to confirm complete Hgb binding.
    • Preliminary exchange-transfusion experiments were conducted in rats.

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    Main Results:

    • Successful synthesis of polymer-bound hemoglobin compounds was achieved.
    • Electrophoresis confirmed complete binding of Hgb to the HES polymer.
    • Transfused rats survived for at least 10 hours with HES-Hgb at low hematocrit (<10%).
    • The retention time of Hgb in urine was extended to 12 hours.

    Conclusions:

    • Polymerization of hemoglobin with hydroxyethyl starch significantly enhances its in vivo retention.
    • HES-Hgb solutions show potential as effective blood substitutes with improved pharmacokinetic properties.
    • This approach offers a promising strategy for developing more stable hemoglobin-based oxygen carriers.