Related Experiment Videos
Monoclonal antibodies against immunoglobulin classes as standardizable reagent in B-cell lymphoma and leukemia
From several fusions of mouse myeloma cells X63-Ag8.653 or NS 1 with spleen cells from BALB/c mice immunized with purified IgG or IgM, we produced a series of monoclonal antibodies directed against IgA, IgM and IgG. The specificity of the antibodies was proved in an enzyme linked immunosorbent assay (ELISA) with purified IgA, IgM and IgG respectively. For diagnostic use we tested our antibodies in paraffin, and cryosections, in blood smears and in cytocentrifugates of peripheral blood. As a test system we used the immunoperoxidase staining method. We showed that the corresponding antigenic determinants are resistant to all used methods of material fixation, and that the monoclonals react with the specific antigen in material of normal donors as well as in pathological material.
From several fusions of mouse myeloma cells X63-Ag8.653 or NS 1 with spleen cells from BALB/c mice immunized with purified IgG or IgM, we produced a series of monoclonal antibodies directed against IgA, IgM and IgG. The specificity of the antibodies was proved in an enzyme linked immunosorbent assay (ELISA) with purified IgA, IgM and IgG respectively. For diagnostic use we tested our antibodies in paraffin, and cryosections, in blood smears and in cytocentrifugates of peripheral blood. As a test system we used the immunoperoxidase staining method. We showed that the corresponding antigenic determinants are resistant to all used methods of material fixation, and that the monoclonals react with the specific antigen in material of normal donors as well as in pathological material.