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Monoclonal antibodies that react with epithelial membrane antigen
M G Ormerod1, K Steele, P A Edwards
1Institute of Cancer Research, Royal Cancer Hospital, Haddow Laboratories, Sutton, Surrey.
Previously reported immunohistochemical studies using three monoclonal antibodies reactive with the human milk fat globule membrane, LICR.LON.M8 and HMFG1 and 2 from the ICRF, have shown that their antigenic determinants have similar tissue distributions. These distributions are also similar to those shown by polyclonal sera against epithelial membrane antigen. Using a radioimmunoassay, we have shown that all three monoclonal antibodies react with epithelial membrane antigen. Immunofluorescent staining of cultures of human breast cells with these antibodies demonstrated that they are heterogeneous in their distribution. Although the results emphasized their similarities, differences between the antibodies suggested that they react with different epitopes on the same molecule. The use of these reagents in diagnostic tumor pathology is discussed.
Previously reported immunohistochemical studies using three monoclonal antibodies reactive with the human milk fat globule membrane, LICR.LON.M8 and HMFG1 and 2 from the ICRF, have shown that their antigenic determinants have similar tissue distributions. These distributions are also similar to those shown by polyclonal sera against epithelial membrane antigen. Using a radioimmunoassay, we have shown that all three monoclonal antibodies react with epithelial membrane antigen. Immunofluorescent staining of cultures of human breast cells with these antibodies demonstrated that they are heterogeneous in their distribution. Although the results emphasized their similarities, differences between the antibodies suggested that they react with different epitopes on the same molecule. The use of these reagents in diagnostic tumor pathology is discussed.