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Restriction site bank vectors. II. DNA sequence analysis of plasmid pJRD158
DNA (Mary Ann Liebert, Inc.)
|June 1, 1984
Abstract:
pJRD158 is a small plasmid vector (3903 bp) derived from pBR327 and specifying resistance to ampicillin and tetracycline. It contains 28 unique restriction sites (and 4 nonunique restriction sites) that can be used for cloning. The DNA sequence and computer-assisted restriction site analysis of pJRD158 are reported. Evidence is also presented that suggests a 2-bp revision of the DNA sequence of pBR322 in the RNA primer region.
Insights
This study details the pJRD158 plasmid vector, useful for cloning due to its unique restriction sites. A minor DNA sequence revision in the related pBR322 plasmid is also identified.
Area of Science:
- Molecular Biology
- Plasmid Technology
- Genetic Engineering
Background:
- The pBR327 plasmid is a widely used cloning vector.
- Understanding plasmid DNA sequences and restriction sites is crucial for molecular cloning.
- Accurate sequence data is essential for reliable genetic manipulation.
Purpose of the Study:
- To characterize the pJRD158 plasmid vector, including its DNA sequence and restriction enzyme sites.
- To provide a detailed resource for researchers utilizing pJRD158 in cloning applications.
- To identify potential sequence discrepancies in related plasmid vectors.
Main Methods:
- DNA sequencing of the pJRD158 plasmid.
- Computer-assisted analysis of restriction enzyme recognition sites.
- Comparative sequence analysis with related plasmids like pBR322.
Main Results:
- The pJRD158 plasmid vector (3903 bp) was fully sequenced.
- It possesses 28 unique and 4 nonunique restriction sites, facilitating diverse cloning strategies.
- A 2-bp sequence revision in the RNA primer region of pBR322 was identified.
Conclusions:
- pJRD158 is a well-characterized plasmid vector suitable for molecular cloning.
- The detailed restriction site map aids in designing cloning experiments.
- The identified sequence revision in pBR322 may necessitate re-evaluation of its primer region.