Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diffusion in the aqueous compartment.

A M Mastro, A D Keith

    The Journal of Cell Biology
    |July 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Electron-spin resonance measured molecular diffusion in mammalian cells. Cytoplasmic viscosity primarily affects particle movement, but cell structure becomes more influential under hypertonic conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    THE POPULATION BIOLOGY OF THE BUTTERFLY, EUPHYDRYAS EDITH A VII. HAS E. EDITH A EVOLVED A SERPENTINE RACE?

    Evolution; international journal of organic evolution·2017
    Same author

    Temperature-induced phase transitions in nematode lipids and their influence on respiration.

    Journal of nematology·2009
    Same author

    A potential role for gap junctions in breast cancer metastasis to bone.

    Journal of musculoskeletal & neuronal interactions·2005
    Same author

    Breast cancer metastasis to bone: evolving models and research challenges.

    Journal of musculoskeletal & neuronal interactions·2005
    Same author

    Influence of muscle strength and total work on exercise-induced plasma growth hormone isoforms in women.

    Journal of science and medicine in sport·2003
    Same author

    Strength, workload, anaerobic intensity and the immune response to resistance exercise in women.

    Acta physiologica Scandinavica·2003
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    Area of Science:

    • Biophysics
    • Cell Biology
    • Physical Chemistry

    Background:

    • Molecular diffusion in cells offers insights into cytoplasmic viscosity and structure.
    • Electron-spin resonance (ESR) is a technique used to study molecular motion.

    Purpose of the Study:

    • To measure the diffusion of a small spin label in the cytoplasm of mammalian cells using ESR.
    • To differentiate the effects of viscosity and structure on molecular diffusion within the cell.

    Main Methods:

    • Utilized electron-spin resonance spectroscopy to measure translational and rotational motion of a spin label in the aqueous cytoplasm.
    • Calculated apparent cytoplasmic viscosity from both translational and rotational diffusion data.
    • Investigated molecular diffusion under physiological and hypertonic conditions.

    Related Experiment Videos

    Main Results:

    • The average diffusion constant was 3.3 X 10(-6) cm2/s, higher in growing or cytochalasin B-treated cells.
    • Calculated cytoplasmic viscosity was 2.0-3.0 centipoise, indicating solvent viscosity is a major determinant of movement at 50-100 Å.
    • Under hypertonic conditions, translational motion decreased significantly, while rotational motion changed minimally, suggesting increased cytoplasmic barriers.

    Conclusions:

    • Under physiological conditions, cytoplasmic solvent viscosity is the primary factor governing particle movement.
    • Hypertonic stress increases cytoplasmic barriers and reduces interstitial space without significantly altering aqueous phase viscosity.
    • Cytoplasmic structure plays a crucial role in the diffusion of larger molecules like proteins.