Identification of immunoreactive thyrotropin-releasing hormone in human neoplasia

Immunoreactive TRH (IR-TRH) was identified in extracts derived from seven human cancer tissues, including two oat cell carcinomas. The IR-TRH concentrations ranged from 29-189 pg/g. Tumor IR-TRH exhibited immunological identity with synthetic pyroglu-his-proamide and coeluted with synthetic [3H]TRH after high pressure liquid chromatography on a mu Bondapak C-18 column. These data extend the number of hypothalamic peptide hormones identified in human neoplastic tissues.