Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple JC virus genomes from one patient.

J D Martin, G C Foster

    The Journal of General Virology
    |August 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    JC virus (JCV) isolates from a progressive multifocal leukoencephalopathy patient contained mixed viral genomes. Analysis revealed distinct Type I and Type II JCV DNA variations, suggesting in vivo genomic evolution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Novel application of neutrinos to evaluate U.S. nuclear weapons performance.

    The Review of scientific instruments·2025
    Same author

    Intussusception; a comparison of the infant and adult types.

    The Southern surgeon·2014
    Same author

    Complications of splenectomy.

    The Southern surgeon·2014
    Same author

    Enantioselective ring construction: synthesis of halogenated marine natural spiro[5.5]undecane sesquiterpenes.

    Journal of the American Chemical Society·2012
    Same author

    Pneumatosis intestinalis and mesenteric venous gas - a manifestation of bacterascites in a patient with cirrhosis.

    Journal of postgraduate medicine·2011
    Same author

    Mixed tumors of the parotid gland.

    The New Orleans medical and surgical journal·2010

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • JC virus (JCV) is associated with progressive multifocal leukoencephalopathy (PML).
    • Previous studies isolated JCV from urine and brain tissue of a PML patient.

    Observation:

    • JCV DNA isolates (MAD-7 and MAD-8) were found to be mixtures of distinct viral genomes.
    • Two main genome types (Type I and Type II) were identified based on restriction endonuclease cleavage patterns.
    • MAD-7 DNA contained both Type I and Type II genomes, while MAD-8 DNA was predominantly Type II.

    Findings:

    • Molecular cloning revealed multiple subtypes within Type II genomes, differing from original isolates.
    • Recombinant MAD-8 DNA unexpectedly contained Type I genomes not detected in the original sample.

    Related Experiment Videos

  • Genomic variations were primarily located in the hypervariable regulatory region and PvuII restriction sites.

    • Implications:

    • The in vivo generation of diverse JCV genomes challenges previous assumptions about viral homogeneity.
    • Understanding in vivo viral evolution is crucial for interpreting JCV pathogenesis and developing therapeutic strategies.
    • This study highlights the importance of analyzing viral populations rather than single isolates for accurate genetic characterization.