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Related Experiment Videos

Bone resorption factors in chronic otitis media.

H Moriyama, C C Huang, M Abramson

    Otolaryngology--Head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
    |June 1, 1984
    PubMed
    Summary

    Collagenase in rat chronic otitis media suggests fibroblasts, stimulated by prostaglandin E2 from macrophages, contribute to bone resorption. This highlights fibroblast-epithelial cell interaction in the disease process.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Pathology

    Background:

    • Chronic otitis media involves bone resorption.
    • The role of collagenase and cellular interactions in this process is not fully understood.

    Purpose of the Study:

    • To identify collagenase in rat chronic otitis media.
    • To investigate the role of macrophages, prostaglandin E2, and cell-cell interactions in collagenase production and bone resorption.

    Main Methods:

    • Immunohistochemical staining (peroxidase-antiperoxidase) to detect collagenase.
    • Culture of rat peritoneal macrophages with lipopolysaccharide (endotoxin).
    • Measurement of prostaglandin E2 levels and confirmation by immunofluorescent staining.

    Main Results:

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    • Collagenase was localized in fibroblasts, mononuclear cells, and osteoclasts in bone-resorbing areas.
    • Collagenase was found in fibroblasts adjacent to epithelial basal cells.
    • Endotoxin-activated macrophages produced minimal collagenase but significantly increased prostaglandin E2.
    • Prostaglandin E2 may stimulate fibroblast collagenase production.

    Conclusions:

    • Activated macrophages, via prostaglandin E2, may stimulate fibroblast collagenase production.
    • Fibroblast-epithelial cell interactions appear to enhance bone resorption in chronic otitis media.