Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization.

Area of Science:

• Molecular Biology
• Genetics
• Biochemistry

Background:

• Human purine nucleoside phosphorylase (PNP) is crucial for purine metabolism.
• Previous studies reported the isolation of a cDNA clone for human PNP.

Purpose of the Study:

• To determine the nucleotide sequence of the human PNP cDNA clone.
• To derive and compare the amino acid sequence with known composition.
• To isolate and characterize the genomic organization of the wild-type human PNP gene.

Main Methods:

• Nucleotide sequencing of the cDNA clone.
• Amino acid sequence derivation and comparison.
• Screening of a bacteriophage lambda library using a cDNA probe.
• Isolation and characterization of human PNP genomic clones.

Main Results:

• The nucleotide sequence of the human PNP cDNA was determined.
• The derived amino acid sequence corresponds to a 32 kilodalton protein.
• Genomic clones of the wild-type human PNP gene were isolated.
• The structural organization of the human PNP gene was elucidated.

Conclusions:

• The complete coding sequence and derived protein structure of human PNP are established.
• The genomic structure of the wild-type human PNP gene has been determined, providing insights into its organization.