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Two plasmid-determined restriction and modification systems in Streptococcus lactis.

A Chopin, M C Chopin, A Moillo-Batt

    Plasmid
    |May 1, 1984
    PubMed
    Summary
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    Two restriction and modification systems were identified in Streptococcus lactis strain IL594. Plasmid pIL6 was confirmed to determine one of these systems, impacting bacterial defense mechanisms.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Streptococcus lactis strain IL594 harbors nine plasmids (pIL1-pIL9).
    • Restriction and modification (R-M) systems are crucial for bacterial defense against foreign DNA.

    Purpose of the Study:

    • To investigate the genetic basis of restriction and modification systems in Streptococcus lactis IL594.
    • To identify specific plasmids responsible for these systems.

    Main Methods:

    • Protoplast-induced curing experiments were performed to segregate plasmids.
    • Plasmid cotransfer experiments were utilized to confirm R-M system linkage.

    Main Results:

    • Two distinct restriction and modification systems were detected in S. lactis IL594.

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  • Protoplast curing indicated a link between R-M activity and plasmids pIL6 or pIL7.
  • Plasmid pIL6 was definitively shown to confer a restriction and modification system upon cotransfer.

    • Conclusions:

    • Plasmid pIL6 is responsible for a specific restriction and modification system in Streptococcus lactis IL594.
    • This finding contributes to understanding plasmid-mediated genetic elements in lactic acid bacteria.