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Related Experiment Videos

Strain differences in Shope fibroma virus. An immunopathologic study.

D S Strayer, E Skaletsky, S Sell

    The American Journal of Pathology
    |August 1, 1984
    PubMed
    Summary

    The Shope fibroma virus (SFV) Boerlage strain caused larger tumors and systemic infection in rabbits, unlike the Patuxent strain. Both SFV strains showed similar tumor histology and regression patterns.

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    Area of Science:

    • Veterinary Virology
    • Pathogen Research
    • Immunology

    Background:

    • Shope fibroma virus (SFV) is a poxvirus known to cause fibromas.
    • Previous studies used non-purified SFV strains, potentially confounding results.
    • Understanding strain-specific pathogenicity is crucial for viral disease management.

    Purpose of the Study:

    • To compare the pathogenic effects of plaque-purified Boerlage and Patuxent SFV strains in neonatal rabbits.
    • To evaluate clinical, histological, and viral antigen distribution differences between the two SFV strains.
    • To assess systemic viral dissemination and host response.

    Main Methods:

    • Neonatal rabbits were inoculated with plaque-purified Boerlage or Patuxent SFV strains.
    • Clinical signs, tumor development, and regression were monitored.
    • Histological examination and immunohistologic staining were performed on various tissues.
    • Viral antigen detection was conducted using immunohistochemistry.

    Main Results:

    • Boerlage SFV induced larger tumors and systemic viral spread (spleen, liver, lungs, kidney, thymus) compared to localized tumors with Patuxent SFV.
    • Histologically, tumors from both strains were identical, showing regression 15-20 days post-inoculation.
    • Boerlage strain recipients exhibited extramedullary hematopoiesis, liver necrosis, and thymic cell death; Patuxent recipients were otherwise normal.
    • Viral antigens were detected in tumors and skin for Boerlage SFV, and only in tumors for Patuxent SFV.

    Conclusions:

    • Plaque-purified Boerlage SFV exhibits greater pathogenicity and systemic dissemination than the Patuxent strain in rabbits.
    • Despite systemic spread, rabbits infected with the Boerlage strain tolerated the infection similarly to those with the Patuxent strain.
    • Strain-specific purification and characterization are essential for accurate assessment of viral pathogenesis.

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