Related Experiment Video
Updated: Aug 1, 2026

Large Insert Environmental Genomic Library Production
Published on: September 23, 2009
The genomes of Desulfovibrio gigas and D. vulgaris
Abstract:
Two-dimensional electrophoresis of sequential double-restriction digests showed that the genome of Desulfovibrio gigas compromised 1.63 x 10(6) bp (1.09 x 10(9) Dal) of DNA; an ammonia-limited chemostat population possessed an average of nine genomes per cell and a multiplying batch culture possessed approximately 17 genomes per cell. The genome size of D. vulgaris (Hildenborough) was 1.72 x 10(6) bp (1.14 x 10(9) Dal); a population from an ammonia-limited batch culture contained four genomes per cell. Control digestions and analyses with Escherichia coli GM4 agreed reasonably with published values: a genome size of 3.95 x 10(6) bp and approximately two genomes per cell from a stationary batch culture in glucose minimal medium. Desulfovibrio gigas carried two plasmids of approximately 70 MDal (1.05 x 10(5) bp) and approximately 40 MDal (6 x 10(4) bp); D. vulgaris (Hildenborough) contained one of approximately 130 MDal (1.95 x 10(5) bp). Single plasmids were also detected in a second strain of D. vulgaris and in strain Berre sol of D. desulfuricans but not in 10 other desulfovibrios including representatives of D. desulfuricans, D. vulgaris, D. salexigens and D. africanus.
More Related Videos
Related Concept Videos
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Bacterial Phylum Tenericutes
Viruses of Archaea
Evolution of Microbial Genome
Microbes and Methanogenesis
Deep Sea Microbial Ecology

