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Related Experiment Videos

Genome size and complexity in Azotobacter chroococcum.

R L Robson, J A Chesshyre, C Wheeler

    Journal of General Microbiology
    |July 1, 1984
    PubMed
    Summary

    Azotobacter chroococcum strains possess multiple plasmids influencing genome size. Plasmid content did not affect nitrogen fixation, hydrogenase activity, or resistance, but impacted UV resistance patterns.

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    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Azotobacter chroococcum is a soil bacterium known for nitrogen fixation.
    • Plasmids are extrachromosomal DNA elements that can confer various traits to bacteria.
    • Understanding the role of plasmids in Azotobacter chroococcum is crucial for agricultural and biotechnological applications.

    Purpose of the Study:

    • To investigate the presence and characteristics of plasmids in Azotobacter chroococcum strains.
    • To determine the effect of plasmid content on bacterial phenotypes, including nitrogen fixation, hydrogenase activity, and resistance profiles.
    • To analyze genome sizes and DNA organization in different Azotobacter chroococcum strains.

    Main Methods:

    • Plasmid profiling using gel electrophoresis.

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  • Phenotypic analysis including nitrogen fixation assays, hydrogenase activity tests, carbon source utilization, and resistance testing (antibiotic, heavy metal, UV).
  • Genome size determination using two-dimensional electrophoresis and calculation of genome equivalents per cell.

    • Main Results:

    • Eight Azotobacter chroococcum strains harbored 2-6 plasmids (7-200+ MDal).
    • Curing plasmids from strain MCC-1 did not alter nitrogen fixation, hydrogenase activity, or resistance to common stressors.
    • Genome sizes varied between strains (CW8: 1.78 x 10^6 bp, MDC-1: 1.94 x 10^6 bp), with MDC-1 showing complex UV resistance kinetics potentially linked to its larger plasmid content.

    Conclusions:

    • Plasmid content in Azotobacter chroococcum does not significantly impact core metabolic functions or general resistance.
    • Genome size and DNA organization, particularly in relation to plasmid presence, may influence specific stress responses like UV resistance.
    • Further research is needed to fully elucidate the functional roles of plasmids and their interaction with the bacterial chromosome in Azotobacter species.