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Delayed-type hypersensitivity in mouse hepatitis virus infection in mice
The Japanese Journal of Experimental Medicine
|April 1, 1984
Summary
Delayed-type hypersensitivity in mouse hepatitis virus infection involves mononuclear cell infiltration. Specific T cells mediate this immune response, as shown by adoptive transfer experiments in mice.
Area of Science:
- Immunology
- Virology
- Pathology
Background:
- Mouse hepatitis virus (MHV) infection can induce immune responses.
- Delayed-type hypersensitivity (DTH) is a critical component of cell-mediated immunity.
Purpose of the Study:
- To investigate the role of DTH in MHV infection.
- To identify the immune cells mediating DTH during MHV infection.
Main Methods:
- Intraperitoneal infection of mice with MHV.
- Elicitation of DTH response using virus-infected cell lysate.
- Administration of cyclophosphamide to assess its effect on DTH.
- Adoptive transfer of spleen T cells to H-2 compatible recipients.
Main Results:
- A specific footpad swelling response, indicative of DTH, was observed 8 days post-infection.
- This DTH response involved the infiltration of mononuclear cells.
- Cyclophosphamide treatment prior to infection did not enhance the DTH response.
- Adoptive transfer of spleen T cells (Lyt 2-) from infected mice successfully transferred the DTH response.
Conclusions:
- Cell-mediated immunity, specifically DTH, plays a role in the host response to MHV infection.
- T cells, particularly Lyt 2- T cells, are crucial for mediating the DTH response in MHV-infected mice.