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Related Experiment Videos

Prolactin binding in the developing rat fetal liver.

R Dhanireddy, R E Ulane

    Life Sciences
    |August 13, 1984
    PubMed
    Summary

    Prolactin receptors emerge in fetal rat liver late in gestation. Freezing liver membranes can reveal these prolactin binding sites, enhancing their detectability for research.

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    Area of Science:

    • Endocrinology
    • Developmental Biology
    • Molecular Biology

    Background:

    • Prolactin is a crucial hormone regulating various physiological processes.
    • Understanding prolactin receptor expression during fetal development is key to comprehending its role.

    Purpose of the Study:

    • To investigate the appearance and characteristics of prolactin binding sites in fetal rat liver membranes during late gestation.
    • To assess the impact of membrane preparation techniques, specifically freezing, on prolactin binding.

    Main Methods:

    • Preparation of particulate liver cell membranes from fetal rats (17-21 days gestation).
    • Incubation with radiolabeled human growth hormone ([125I] hGH) to detect prolactin binding.
    • Assessment of specific binding using excess ovine prolactin and Scatchard analysis.

    Main Results:

    • Specific prolactin binding was detected only in membranes from 21-day gestation fetuses.
    • Freezing membranes from 20-day gestation fetuses unmasked prolactin binding sites.
    • Freezing significantly increased prolactin binding in 21-day gestation membranes by approximately 45%.

    Conclusions:

    • Prolactin receptors are expressed in the fetal rat liver during the final stages of gestation.
    • Membrane freezing is a critical step that can enhance the detection of prolactin binding sites, suggesting they may be initially inaccessible.

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