[Characteristics of kinetoplastic DNA from Leptomonas pessoai]

The kinetoplast (mitochondrial) DNA from trypanosomatid Leptomonas pessoai represents a network, composed of mini-circles heterogeneous in base sequence and homogeneous maxi-circles and thus has the main structural features in common with DNAs from kinetoplasts of other Trypanosomatidae. The size of mini-circular molecules of DNA is 1,35 kilobase pairs (kbp) and that of maxicircular molecules-30,9 kbp. Based on the data of single and double restriction cleavages the physical map of the maxi-circular molecules was constructed for the endonucleases BamHI, BglII, BspI , HindIII, MspI, SalGI and PstI.