Novel ligand specificity of pituitary vasopressin receptors in the rat
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Updated: Aug 15, 2026

Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
Abstract:
The ligand specificities of sites binding tritium-labeled arginine vasopressin (3H-AVP) were investigated in membrane preparations of rat anterior pituitary gland and liver. Labeled AVP interacted with high-affinity, low-capacity binding sites in liver as well as in pituitary membrane fractions. Binding displacement studied showed that AVP was a potent ligand for liver (KI = 0.23 nM) and pituitary (KI = 0.4 nM) receptors. Oxytocin and the antidiuretic (V2) agonist dDAVP had low affinities (KI greater than 10 nM) both for liver and pituitary binding sites. The pressor (V1) antagonist d(CH2)5Tyr(Me)AVP was equipotent with AVP in liver membranes, but was 1,000-fold less potent on pituitary receptors. Another V1 antagonist, dPenTyr(Me)AVP, displaced 3H-AVP with KI values of 3 and 8 nM from pituitary and liver receptors, respectively. These data are in reasonable agreement with recent studies of the in vitro biological activities of the analogs tested, and suggest that the ligand specificity of rat pituitary AVP receptors is distinct from those of previously characterized V1, V2 and oxytocin binding sites in the rat.
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