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Nucleotide sequence of the maize transposable element Mul.

R F Barker, D V Thompson, D R Talbot

    Nucleic Acids Research
    |August 10, 1984
    PubMed
    Summary

    Researchers characterized the Mul insertion element in maize, crucial for Robertson's Mutator activity. This element exhibits complex internal repeats and open reading frames, suggesting a role in maize genetic instability.

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    Area of Science:

    • Plant Molecular Biology
    • Genetics
    • Transposable Elements

    Background:

    • Robertson's Mutator is a maize genetic system associated with high mutation rates.
    • Transposable elements, like Mul, are mobile DNA sequences that can alter gene function and genome structure.

    Purpose of the Study:

    • To characterize the DNA sequence and structure of the Mul insertion element.
    • To investigate the molecular organization of Mul and its relationship to Mutator activity in maize.

    Main Methods:

    • DNA cloning and sequencing of the Adhl-S3034 allele.
    • Bioinformatic analysis to identify structural features like repeats and open reading frames (ORFs).
    • Comparative analysis of Mul sequences in different Mutator maize lines.

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    Main Results:

    • The cloned Mul element is 1367 bp long, with direct repeats at the insertion site and inverted terminal repeats.
    • Mul contains two internal direct repeats and four open reading frames (ORFs), organized into two distinct halves.
    • A central region of Mul shows low GC content and contains a potential eukaryotic promoter signal (TAATA).
    • Related Mul sequences in Mutator maize are similarly organized, with some lines containing larger insertions.

    Conclusions:

    • The Mul element possesses a complex internal structure with features suggestive of regulatory or functional domains.
    • The conserved organization of Mul elements in Mutator maize supports their role in generating genetic instability.
    • The presence of a potential promoter sequence within Mul warrants further investigation into its transcriptional activity.