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Updated: Aug 18, 2026

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
07:02

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)

Published on: September 14, 2010

A variant of human cytomegalovirus derived from a persistently infected culture

T Furukawa

    Virology
    |August 1, 1984
    PubMed

    Insights

    Persistent human cytomegalovirus (HCMV) infection in osteogenic sarcoma cells is linked to a balance between viral release and cell growth. A variant HCMV strain with deleted repeat sequences likely causes this persistent infection.

    Area of Science:

    • Virology
    • Cell Biology
    • Molecular Biology

    Background:

    • Human cytomegalovirus (HCMV) can establish persistent infections in host cells.
    • Osteogenic sarcoma cells (HOS) are susceptible to HCMV infection.

    Purpose of the Study:

    • To investigate the mechanism of persistent HCMV infection in HOS cells.
    • To characterize the viral and cellular factors involved in maintaining the persistent infection.

    Main Methods:

    • Infection of HOS cells with HCMV.
    • Analysis of viral properties (temperature sensitivity, interfering potential).
    • Hybridization experiments using labeled probes to detect viral DNA alterations.

    Main Results:

    • HCMV infection of HOS cells led to a persistent infection.
    • Persistent viruses were not temperature sensitive or defective interfering particles.
    • Deletion of repeat sequences (fragments Q and O) was observed in the DNA of HCMV from persistent cultures.

    Conclusions:

    • Persistent HCMV infection in HOS cells is likely mediated by a variant viral strain.
    • The variant HCMV possesses deleted repeat sequences, contributing to the persistent infection mechanism.

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