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Related Experiment Videos

Cyclic nucleotides and their relationship to complement-component-C2 synthesis by human monocytes.

D Lappin, D W Riches, B Damerau

    The Biochemical Journal
    |September 1, 1984
    PubMed
    Summary

    This study reveals two key signals regulating monocyte complement component C2 production. Increased cyclic AMP inhibits C2, while cyclic GMP and possibly calcium influx stimulate it.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Monocytes play a crucial role in the immune system, including the production of complement components like C2.
    • Cyclic nucleotides, cyclic AMP and cyclic GMP, are important intracellular messengers involved in various cellular processes.

    Purpose of the Study:

    • To investigate the role of cyclic nucleotides in regulating complement component C2 production by monocytes.
    • To identify the specific signaling pathways involved in C2 synthesis modulation.

    Main Methods:

    • Monocytes were treated with various agents known to affect cyclic nucleotide levels (histamine, PGE2, cholera toxin, NaF, phosphodiesterase inhibitors, adrenergic and cholinergic ligands, prostaglandin synthetase inhibitors).
    • Changes in cyclic AMP and cyclic GMP levels were measured over time.

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  • Complement component C2 production was quantified following these treatments.

    • Main Results:

    • Histamine and PGE2 rapidly increased cyclic AMP, while cholera toxin, NaF, and phosphodiesterase inhibitors caused sustained increases.
    • Adrenergic, cholinergic ligands, and imidazole decreased cyclic AMP and increased cyclic GMP.
    • Increased cyclic AMP levels correlated with decreased C2 production, and decreased cyclic AMP correlated with increased C2 production.
    • Agents increasing cyclic GMP alone did not affect C2 production, suggesting a distinct role.

    Conclusions:

    • Two transmembrane signals regulate monocyte C2 production: adenylyl cyclase activation (inhibitory) and potentially Ca2+ influx (stimulatory).
    • The observed changes in cyclic nucleotides and C2 production suggest complex signaling crosstalk in immune cells.