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Inverted repeat sequences flank a Bacillus thuringiensis crystal protein gene.

J W Kronstad, H R Whiteley

    Journal of Bacteriology
    |October 1, 1984
    PubMed
    Summary

    Two inverted repeat DNA sequences, IR2150 and IR1750, were found near the crystal protein gene in Bacillus thuringiensis. These sequences are present on both plasmids and chromosomes, correlating with the crystal protein gene

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Bacillus thuringiensis subsp. kurstaki HD73 contains a 75-kilobase plasmid encoding a crystal protein gene.
    • Inverted repeat (IR) DNA sequences play roles in DNA replication, recombination, and gene regulation.

    Purpose of the Study:

    • To investigate the presence and organization of inverted repeat DNA sequences flanking the crystal protein gene in Bacillus thuringiensis.
    • To determine the copy number and orientation of these sequences on plasmids and chromosomes.

    Main Methods:

    • Restriction mapping of the plasmid DNA surrounding the crystal protein gene.
    • DNA hybridization experiments to detect and locate IR sequences on plasmids and chromosomes.
    • Analysis of DNA from multiple Bacillus thuringiensis strains.

    Main Results:

    • Two sets of inverted repeat sequences, IR2150 and IR1750, were identified flanking the crystal protein gene.
    • Multiple copies of IR2150 and IR1750 were found on the chromosome of B. thuringiensis subsp. kurstaki HD73.
    • A strong correlation was observed between the presence of these IR sequences and the crystal protein gene on plasmids from 14 different B. thuringiensis strains.

    Conclusions:

    • The IR2150 and IR1750 sequences are significant genetic elements associated with the crystal protein gene in Bacillus thuringiensis.
    • These sequences are likely involved in the maintenance or regulation of the crystal protein gene, potentially through plasmid-chromosome interactions or gene amplification.

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